Provides instructions for constructing Singleplexed Gene and (TotalSeq™️-C) Protein Expression libraries.
For use with:
- GEM-X Flex Sample Preparation v2 Kit PN-1000781
- GEM-X Flex GEM & Library Kit, 4 rxns PN-1000782
- GEM-X Flex Hybridization & Wash Kit, 24 rxns PN-1000789
- GEM-X Flex Human Transcriptome Probe Kit, 4 samples PN-1000783
- GEM-X Flex Mouse Transcriptome Probe Kit, 4 samples PN-1000784
- GEM-X Flex Gel Bead Kit, 4 rxns PN-1000790
- Fixed RNA Feature Barcode Multiplexing Kit, 64 rxns PN-1000628
- GEM-X Flex Gene Expression Chip Kit, 4 chips PN-1000791
- Dual Index Kit TS Set A, 96 rxns PN-1000251
- Dual Index Kit TN Set A, 96 rxns PN-1000250
Important
GEM-X FX chips are compatible with Chromium X and iX instruments; GEM-X FX chips are incompatible with Xo instruments. To run the GEM-X FX chips in the Chromium X or iX instrument, firmware version 2.0.0 or higher is required; however the most updated version is recommended for best performance.
Step by step video demo
GEM-X Chip Assembly, Loading & GEM Generation