Support homeSpatial Gene Expression for Fresh Frozen


Find resources from protocols to free-to-use analysis tools.
Experimental Design & Planning

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial protocol.

Tissue Prep

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Tissue Preparation Guide.

Tissue Staining

Methanol fix, stain, and image tissue following our Demonstrated Protocol.

Tissue Optimization

Optimize permeabilization conditions for your fresh frozen tissue sections by following our step-by step User Guide.


For imaging hardware, general image acquisition and analysis guidelines, and example images, refer to our Technical Note.

Library Construction

Construct a sequencing-ready Visium spatial library from your fresh frozen tissue sections following our step-by-step User Guide.

Targeted Gene Expression (Optional)

Use pre-designed panels for targeted gene expression, followed by sequencing and data analysis.


Sequence your Visium spatial library as per our provided recommendations.

Software Analysis

Analyze your Visium spatial data using our pipelines and visualization tools.