List of human FFPE tissue types that were validated in-house with Xenium In Situ Gene and Protein Expression with Cell Segmentation Staining (CG000819). Xenium Gene and Protein Expression success depends on stain quality, which in turn, depends on sample quality and tissue cell type composition. Tissues tested in-house were acquired from biobanks. All tissues listed below were processed per our standard documentation following FFPE Tissue Preparation (CG000578) and Xenium In Situ Gene and Protein Expression (CG000819).
Some tissues were tested with multiple tissue states. Example datasets can be found here.
| Tissue | Disease state | Primary Diagnosis | Important Considerations |
|---|---|---|---|
| Tonsil | Diseased | Lymphoid hyperplasia | |
| Lymph node | Diseased / non-diseased | Diffuse Large B cell Lymphoma, Follicular Lymphoma | |
| Breast | Diseased | IDC triple positive / negative, Invasive ductal carcinoma, Infiltrating lobular carcinoma | |
| Colon | Diseased / non-diseased | Adenocarcinoma | |
| Appendix | Diseased / non-diseased | Crohn’s disease/ Invasive Mucinous Adenocarcinoma | |
| Lung | Diseased / non-diseased | Inflammation, Adenocarcinoma | |
| Kidney | Diseased / non-diseased | Clear cell carcinoma | |
| Skin | Diseased / non-diseased | Melanoma, Basal cell carcinoma | Even though skin can be prone to detachment, recommend following the User Guide as written for decrosslinking conditions. |
| Liver | Diseased / non-diseased | Hepatocellular carcinoma | |
| Ovary | Diseased | Serous carcinoma | |
| Spleen | Diseased | Inflammation | |
| Small intestine | Diseased / non-diseased | Colorectal Cancer | |
| Pancreas | Non-diseased | ||
| Prostate | Diseased | Adenocarcinoma | |
| Brain | Diseased | Glioblastoma |