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In Situ Gene Expression

Updated custom probe resuspension. Refer to the Xenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplication User Guide (CG000582 Rev E) for details
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What is In Situ Gene Expression?

The Xenium In Situ enables high-throughput subcellular mapping of 100s of RNA targets, revealing new insights into cellular structure and function. The platform includes a versatile and easy-to-use instrument, sensitive and specific chemistry, and a diverse menu of customizable panels.

Get startedFind User Guides

Documentation

Find resources, from protocols to free-to-use analysis tools. Workflow varies based on your sample type. Xenium In Situ Gene Expression currently supports both fresh frozen and FFPE samples.

Experimental Design & Planning

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Xenium Gene Expression.

Panel Design

Learn about the pre-designed Gene Expression panels and engage with 10x design experts to design, order, and manufacture Xenium Custom Genes.

Tissue Prep for FFPE

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for FFPE.

Tissue Prep for Fresh Frozen

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for Fresh Frozen.

Assay

Prepare tissue sections on Xenium slides for on-instrument analysis by following our step-by-step User Guide/Protocols.

Instruments

Refer to instrument-specific documents for seamless instrument operation.

Software Analysis

Analyze your Xenium in situ data using our pipelines and visualization tools.

Safety Data Sheets

Provides safety information for Xenium.

Datasets

Access free, publicly available data from a number of different sample types generated with Xenium In Situ solutions.

View datasets
Datasets

Videos

Xenium In Situ Tissue Preparation video
Technical Overview series
Xenium In Situ Tissue Preparation
Xenium In Situ Assay Protocols - Best Practices video
Technical Overview series
Xenium In Situ Assay Protocols - Best Practices
Q&A

Find answers to common technical questions, from sample prep through data analysis, about In Situ workflows.

Q&A