Cell surface proteins can be labeled using a specific protein binding molecule, such as an antibody conjugated to a Feature Barcode oligonucleotide. This protocol provides guidance for antibody-oligonucleotide conjugation and outlines cell surface protein labeling for use with Single Cell RNA sequencing protocols with Feature Barcoding technology. This protocol also provides guidance for enriching labeled cells using Fluorescence Activated Cell Sorting (FACS).

To design a custom conjugate oligonucleotide, use a sequence from the Feature Barcode inclusion list (CG000193). Please refer to the following article for details: How do I use the Feature Barcode Whitelist?

FOR USE WITH:

Universal 3’ Gene Expression

• GEM-X Universal 3' Gene Expression v4 4-plex On-chip Multiplexing (OCM) with Feature Barcode technology for Cell Surface Protein (CG000769)
• Chromium GEM-X Single Cell 3' Reagent Kits v4 with Feature Barcode technology for Cell Surface Protein (CG000732)

Universal 5’ Gene Expression

• GEM-X Universal 5' Gene Expression v3 4-plex On-chip Multiplexing (OCM) with Feature Barcode technology for Cell Surface Protein (CG000771)
• GEM-X Universal 5' Gene Expression v3 4-plex On-chip Multiplexing (OCM) with Feature Barcode technology for CRISPR Screening & Cell Surface Protein (CG000773)
• Chromium GEM-X Single Cell 5' Reagent Kits v3 with Feature Barcode technology for Cell Surface Protein (CG000734)
• Chromium GEM-X Single Cell 5' Reagent Kits v3 with Feature Barcode technology for CRISPR Screening & Cell Surface Protein (CG000734)