Dissecting mechanisms of immune tolerance with single cell multiomic analysis

Researchers at the Geisel School of Medicine at Dartmouth (Lebanon, NH, USA) provide evidence that V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA) serves as the earliest checkpoint regulator of T-cell tolerance. VISTA promotes T-cell quiescence and balances cell types and states within the naïve CD4+ T-cell compartment. By examining changes in transcriptome, T-cell repertoire, and open chromatin, the Chromium Single Cell Immune Profiling and Chromium Single Cell ATAC solutions helped reveal the role of VISTA in immune tolerance.