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1k 1:1 Mixture of Human HEK293T and Mouse NIH3T3 Cells, 3’ LT v3.1

Universal 3' dataset analyzed using Cell Ranger 6.0.0

Number of samples on this datasets page1

1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells obtained by 10x Genomics from ATCC were used in a classic experiment to demonstrate single cell behavior.

Libraries were generated following the Chromium Next GEM Single Cell 3' LT Reagent Kits v3.1 (Dual Index) User Guide (CG000399) and sequenced on Illumina NovaSeq 6000.

Sequencing Depth: 57,129 mean reads per cell

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)

Analysis Parameters Used: Run with --expect-cells=1000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Additional Applications

Low Throughput

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Mouse, Human

Anatomical Entity

Embryonic Kidney, Embryo

Preservation Method

Fresh Frozen

Cells or Nuclei

Cells

Selected Cell Types

fibroblast

Biomaterial Type

Cell Line

Cell/Nuclei Count

808

Donor Count

2

Date Published

2021-03-31