
Peripheral blood mononuclear cells (PBMCs) from a CMV seropositive donor, were obtained through a collaboration. Cells were stained with a panel of 12 dCODE™ Dextramer® reagents (Immudex) and 17 TotalSeq™-C antibodies (BioLegend). See details in the Application Note [“Redefining Cellular Phenotyping: Comprehensive Characterization and Resolution of the Antigen-Specific T Cell Response”](/resources/document-library/740da7).

The gene expression and cell surface protein libraries from the sorted ([vdj_v1_hs_sorted_cmvpos_5gex_protein](https://support.10xgenomics.com/single-cell-vdj/datasets/3.0.2/vdj_v1_hs_sorted_cmvpos_5gex_protein)) and unsorted ([vdj_v1_hs_unsorted_cmvpos_5gex_protein](https://support.10xgenomics.com/single-cell-vdj/datasets/3.0.2/vdj_v1_hs_unsorted_cmvpos_5gex_protein)) samples were aggregated using Cell Ranger aggr.

8,549 total cells detected
1,303 median genes per cell and 4,431 median UMI counts per cell detected
\~73,000 read pairs per cell pre-normalization; \~53,000 reads per cell post-normalization


Summary HTML

Loupe Browser file