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Flash-Frozen Cortex, Hippocampus, and Ventricular Zone from Embryonic Mouse Brain (E18)

Epi ATAC dataset analyzed using Cell Ranger ATAC 1.1.0

Number of samples on this datasets page1

Flash-frozen cortex, hippocampus, and ventricular zone from embryonic mouse brain (E18). ~7,700 transposed nuclei were loaded and 4,580 nuclei were recovered.

Libraries were sequenced on Illumina NovaSeq with approximately 49k read pairs per cell:

  • 50 bp Read 1
  • 8 bp i7 index (sample index)
  • 16 bp i5 index (10x Barcode)
  • 49 bp Read 2

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Epi ATAC

Software

Cell Ranger ATAC

Subpipeline

cellranger-atac count

10x Instrument(s)

Chromium Controller

Species

Mouse

Anatomical Entity

Brain, Subventricular Zone, Hippocampus, Cortex

Preservation Method

Fresh Frozen

Cells or Nuclei

Nuclei

Selected Cell Types

Neurons

Biomaterial Type

Specimen from Organism

Cell/Nuclei Count

4,580

Donor Count

1

Date Published

2019-04-16