Human dissociated lymph node tumor cells of a 19-year-old male Hodgkin’s Lymphoma patient were obtained by 10x Genomics from Discovery Life Sciences. Whole transcriptome libraries were generated with Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) User Guide (CG000315). The targeted libraries were generated using the Targeted Gene Expression Reagent Kits User Guide (CG000293) and Human Pan-Cancer Panel Reagent (PN-1000247) and sequenced on an Illumina NovaSeq 6000.
Targeted Gene Expression Library
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Sequencing Depth: 11,332 reads per cell
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Paired-end (28 x 90) dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i7 index: 10 cycles i5 index: 10 cycles Read 2: 90 cycles (transcript)
Key metrics were:
- Cells detected: 3,049
- Read mapped confidently to targeted transcriptome: 87.2%
- Total targeted genes detected: 1,165