Human B cells isolated from a healthy female donor pre- and post- influenza vaccination were obtained by 10x Genomics from Discovery Life Sciences. Post-vaccination sample was obtained 14 days after vaccination.

Gene expression and BCR Amplified libraries were generated from 16,400 cells (85% viable, expected recovery 8,500 cells) as described in the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide (CG000331 Rev A) and sequenced on an Illumina NovaSeq 6000. Libraries from the pre- and post-vaccination B cells were analyzed using Cell Ranger _aggr_.


Inputs CSV

VDJ reference

Feature Reference CSV (used to generate aggr inputs)

Target Panel CSV

Multi - Summary HTML

Gene Expression - Feature / cell matrix HDF5 (filtered)

Gene Expression - Feature / cell matrix (filtered)

Gene Expression - Feature / cell matrix HDF5 (raw)

Gene Expression - Feature / cell matrix (raw)

Gene Expression - Clustering analysis

Gene Expression - Summary JSON

Gene Expression - Loupe Browser file

VDJ Ig - Clonotype info (CSV)

VDJ Ig - Clonotype consensus annotations (CSV)

VDJ Ig - Clonotype consensus FASTA

VDJ Ig - Filtered contig annotations (CSV)

VDJ Ig - Loupe V(D)J Browser file

VDJ TCR - Clonotype info (CSV)

VDJ TCR - Clonotype consensus annotations (CSV)

VDJ TCR - Clonotype consensus FASTA

VDJ TCR - Filtered contig annotations (CSV)

VDJ TCR - Loupe V(D)J Browser file

Summary

Files

Chromium Controller

Chromium

Gene Expression

Epigenomic Profiling

Immune Profiling

Protein co-detection

Compact system for multiomic analysis of hundreds to tens of thousands of single cells.

GEM-X technology-powered assays with whole transcriptome coverage to analyze immune diversity 

Universal 5'

Chromium X Series

Advanced system for multiomic analysis of hundreds to millions of cells.

5' gene expression alongside V(D)J repertoire profiling and antigen specificity of T and B cells.

Cell Ranger

Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, cell surface protein expression, or CRISPR edits in each cell.

Cell Surface Protein

CRISPR Screening

Sample multiplexing

Reveal the full complexity of cellular diversity, <em>cell by cell</em>

Single Cell Gene Expression

3' gene expression profiling at scale with single cell resolution.

Chromium Single Cell Immune Profiling provides a multiomic solution to your immunology questions. Analyze full-length V(D)J sequences for paired B-cell or T-cell receptors, cell surface protein expression, antigen specificity, and/or CRISPR edits, along with gene expression, all from a single cell.

Antigen Specificity

Multiomic profiling of immune diversity, <em>cell by cell</em>

Single Cell Immune Profiling

Profile a defined set of transcripts from single cells or tissue sections with Targeted Gene Expression. Reduce costs while increasing the number of samples, or increasing sequencing depth with customizable, comprehensive gene panels.

Discontinuation of Targeted Gene Expression

Chromium Connect

Automated system for single cell partitioning, barcoding, and library preparation.

Targeted Gene Expression

Visium

Profiling of defined transcript sets from single cells or fresh frozen tissue sections.

Analyze single cell gene expression data with a set of free, easy-to-use analysis pipelines.

Human B cells from a healthy donor pre- and post- flu vaccination (v2)

_Depending on your experimental goals, additional Library Construction Kits (PN-1000190) may be required. Please click [here](https://kb.10xgenomics.com/hc/en-us/articles/360048287712-When-do-I-need-an-additional-Library-Construction-Kit-PN-1000190-to-run-my-experiment-with-Next-GEM-5-v2-reagent-kits) for further guidance._

FOR USE WITH:

- Chromium Next GEM Single Cell 5' Kit v2, 16 rxns PN-1000263
- Chromium Next GEM Single Cell 5' Kit v2, 4 rxns PN-1000265
- Library Construction Kit, 16 rxns PN-1000190
- Chromium Single Cell Human TCR Amplification Kit, 16 rxns PN-1000252
- Chromium Single Cell Human BCR Amplification Kit, 16 rxns PN-1000253
- Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns PN-1000254
- Chromium Single Cell Mouse BCR Amplification Kit, 16 rxns PN-1000255
- Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286
- Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287
- Dual Index Kit TT Set A, 96 rxns PN-1000215


Provides instructions for constructing libraries using the Chromium V(D)J Single Cell 5' Reagent Kit (v2).

Human B cells from a healthy donor pre- and post- flu vaccination (v2)

Assess data quality

Visualize and explore data