5 µm section from squamous cell carcinoma of human cervical cancer. FFPE tissue purchased from Discovery Life Sciences.
- Block C00084155.1a
- Cervical Cancer, Invasive. Squamous cell carcinoma T1bN0M0 IB
- Brightfield camera, Magnification: 20x
- Sequencing instrument: Illumina NovaSeq 6000, flow cell HVFG7DSXY (lanes 1-4)
- Sequencing depth: 73,046 reads per spot
- Sequencing configuration: 28 bp read 1 (16 bp Visium spatial barcode, 12 bp UMI), 50 bp read 2 (transcript), 10 bp i7 sample barcode and 10 bp i5 sample barcode
- Dual-Index set: SI-TS-E5
- Slide: V10L13-019
- Area: A1
Key metrics were:
- Spots detected under tissue: 2,781
- Median genes per spot: 7,276
- Median UMI counts per spot: 26,050