Describes methods for decalcifying and preparing human and mouse bone tissue samples for use in Xenium v1 In Situ Gene Expression assays. 

Analysis of Bone Tissue using Xenium v1 In Situ Gene Expression Assay

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for FFPE.

Tissue Prep for FFPE

The Xenium In Situ enables high-throughput subcellular mapping of 100s of RNA targets, revealing new insights into cellular structure and function. The platform includes a versatile and easy-to-use instrument, sensitive and specific chemistry, and a diverse menu of customizable panels.

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Xenium Gene Expression.

Experimental Design

Experimental Design & Planning

Learn about the pre-designed Gene Expression panels and engage with 10x design experts to design, order, and manufacture Xenium Custom Genes.

Panel Design

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for Fresh Frozen.

Tissue Prep for FF

Tissue Prep for Fresh Frozen

Prepare tissue sections on Xenium slides for on-instrument analysis by following our step-by-step User Guide/Protocols.

Assay

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Analyze your Xenium in situ data using our pipelines and visualization tools.

Software Analysis

Safety Data Sheets

Xenium Analyzer

原位基因表达分析

Overview

User Guides

Workflow Documentation

Datasets

Videos

Service Providers

In Situ Gene Expression

<>{'NEW Xenium Cell Segmentation Add-On Kit User Guide. NEW Storage Conditions for Xenium slides. Refer to '}<Anchor href={'/support/in-situ-gene-expression/documentation/steps/assay/xenium-in-situ-gene-expression-probe-hybridization-ligation-amplification'}>{'Xenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplication User Guide (CG000582 Rev F)'}</Anchor>{' for details.'}</>

What is In Situ Gene Expression?

Find resources, from protocols to free-to-use analysis tools. Workflow varies based on your sample type. Xenium In Situ Gene Expression currently supports both fresh frozen and FFPE samples.

Documentation

Access free, publicly available data from a number of different sample types generated with Xenium In Situ solutions.

Xenium In Situ Tissue Preparation

Xenium In Situ Assay Protocols - Best Practices

Find answers to common technical questions, from sample prep through data analysis, about In Situ workflows. 

Analysis of Bone Tissue using Xenium v1 In Situ Gene Expression Assay

Technical Note, CG000816

CG000816_Xenium_Bone_Compatibility_TechNote_RevA.pdf