10x Genomics Support/Single Cell Gene Expression/Documentation/Sample Prep/

Neutrophil Analysis in 10x Genomics Single Cell Gene Expression Assays

  • CG000444_Neutrophil Analysis_TN_RevA .pdf

    Technical Note, CG000444

    CG000444_Neutrophil Analysis_TN_RevA .pdf

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Neutrophils are the most abundant white blood cells in circulation. They are the first responders of the innate immune system that release cytotoxic compounds to kill bacteria and phagocytose foreign particles. Analysis of neutrophils and granulocytes is challenging in single cell transcriptomic data due to the low RNA content in these cells along with relatively high levels of RNases and other inhibitory compounds. Furthermore, neutrophils are sensitive to degradation after collection, requiring careful sample handling for preservation. This Technical Note describes the successful isolation and analysis of neutrophil populations from whole blood.

Document Type
Technical Note

Last Modified
October 25, 2021