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Universal 3' Gene Expression

Universal 3’

Chromium Single Cell Universal 3’ Gene Expression provides single cell transcriptome 3' gene expression alongside the detection of surface protein expression in tens of thousands of cells.

Workflow Overview

Universal 3' Gene Expression workflow, from experimental design through data analysis

Experimental Design & Planning

Planner, CG000748

Protocol Planner

Workflow overview and additional reagents for workflow. Before starting the experiment, consult these documents for protocol

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Sample Preparation

Handbook, CG000053

Cell Preparation Handbook

General guidance on sample preparation best practices

Specific Sample Preparation Documents

General guidance on specific sample preparation protocols

Demonstrated Protocol, CG000149

Cell Surface Protein Labeling Demonstrated Protocol

Label cells with antibody-oligonucleotide conjugates for use with 10x Genomics Single Cells Protocols with Feature Barcode technology for Cell Surface Protein

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Library Construction

Library Construction
User Guide, CG000731

Chromium GEM-X Single Cell 3' Reagent Kits v4

Construct 3' Gene Expression libraries

User Guide, CG000732

Chromium GEM-X Single Cell 3' Reagent Kits v4 with Feature Barcode technology for Cell Surface Protein

Construct 3' Gene Expression and Cell Surface Protein libraries

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Sequencing

Handbook, CG000809

Sequencing Handbook

Provides guidance and best practices for sequencing 10x Genomics libraries.

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Data analysis

Cell Ranger

Analyze single cell gene expression data with a set of free, easy-to-use analysis pipelines.

Cloud Analysis

Analyze 10x Genomics data quickly and securely in the cloud.

Loupe Browser

Explore 10x Genomics data with our powerful visualization software. Compatible with single cell and spatial products.

How-to videos

Other resources

Company

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