Universal 3’
Workflow Overview

Experimental Design & Planning
Protocol Planner
Workflow overview and additional reagents for workflow. Before starting the experiment, consult these documents for protocol
Sample Preparation
Specific Sample Preparation Documents
General guidance on specific sample preparation protocols
Cell Surface Protein Labeling Demonstrated Protocol
Label cells with antibody-oligonucleotide conjugates for use with 10x Genomics Single Cells Protocols with Feature Barcode technology for Cell Surface Protein
Library Construction
Chromium GEM-X Single Cell 3' Reagent Kits v4
Construct 3' Gene Expression libraries
Chromium GEM-X Single Cell 3' Reagent Kits v4 with Feature Barcode technology for Cell Surface Protein
Construct 3' Gene Expression and Cell Surface Protein libraries
Sequencing
Sequencing Handbook
Provides guidance and best practices for sequencing 10x Genomics libraries.
Data analysis
Cell Ranger
Analyze single cell gene expression data with a set of free, easy-to-use analysis pipelines.
Cloud Analysis
Analyze 10x Genomics data quickly and securely in the cloud.
Loupe Browser
Explore 10x Genomics data with our powerful visualization software. Compatible with single cell and spatial products.
How-to videos
Other resources

Knowledge base Q&A
Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

Datasets
Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

Publications
Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.


