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1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

Single Cell Gene Expression dataset analyzed using Cell Ranger 1.0.0

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1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells.

This is a classic human-mouse mixture experiment to demonstrate single cell behavior.

  • 1,387 cells detected
  • Sequenced on Illumina NextSeq 500 with approximately 80,000 reads per cell
  • 98bp read1 (transcript), 8bp I5 sample barcode, 14bp I7 GemCode barcode and 10bp read2 (UMI)
  • Analysis run with --cells=1200

This dataset is licensed under the Creative Commons Attribution license.