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10k 1:1 Mixture of Human HEK293T and Mouse NIH3T3 Cells, 3’ v3.1

Universal 3' dataset analyzed using Cell Ranger 6.0.0

Number of samples on this datasets page1

1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells obtained by 10x Genomics from ATCC were used in a classic experiment to demonstrate single cell behavior.

Libraries were generated following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) User Guide (CG000315) and sequenced on Illumina NovaSeq 6000.

Sequencing Depth: 64,763 mean reads per cell

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)

Analysis Parameters Used: Run with --expect-cells=10000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human, Mouse

Anatomical Entity

Embryonic Kidney, Embryo

Preservation Method

Fresh Frozen

Cells or Nuclei

Cells

Selected Cell Types

fibroblast

Biomaterial Type

Cell Line

Cell/Nuclei Count

9,383

Donor Count

2

Date Published

2021-03-31