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2k Brain Nuclei from an Adult Mouse (>8 weeks)

Universal 3' dataset analyzed using Cell Ranger 2.1.0

Number of samples on this datasets page1

Nuclei from a combined cortex, hippocampus and sub ventricular zone of an adult mouse (>8 weeks).

  • 2,352 nuclei detected
  • Sequenced on Illumina HiSeq4000 with approximately 48,000 reads per cell
  • 26bp read1 (16bp Chromium barcode and 10bp UMI), 98bp read2 (transcript), and 8bp I7 sample barcode
  • Analysis run with --expect-cells=2000
  • Analysis run with reference version 1.2.0

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Mouse

Anatomical Entity

Brain, Cortex, Subventricular Zone, Hippocampus

Cells or Nuclei

Nuclei

Selected Cell Types

Neurons

Biomaterial Type

Specimen from Organism

Cell/Nuclei Count

2,352

Donor Count

1

Date Published

2017-11-08