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3k PBMCs from a Healthy Donor

Universal 3' dataset analyzed using Cell Ranger 1.0.0

Number of samples on this datasets page1

Peripheral blood mononuclear cells (PBMCs) from a healthy donor (same donor as pbmc6k).

PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

  • 2,700 cells detected
  • Sequenced on Illumina NextSeq 500 with ~69,000 reads per cell
  • 98bp read1 (transcript), 8bp I5 sample barcode, 14bp I7 GemCode barcode and 10bp read2 (UMI)
  • Analysis run with --cells=3000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger run

10x Instrument(s)

Chromium Controller

Species

Human

Anatomical Entity

Blood

Disease State

healthy

Cells or Nuclei

Cells

Selected Cell Types

PBMCs

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

2,691

Donor Count

1

Date Published

2016-05-26