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Human Cervical Cancer (FFPE)

Spatial Gene Expression dataset analyzed using Space Ranger 1.3.0

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5 µm section from squamous cell carcinoma of human cervical cancer. FFPE tissue purchased from Discovery Life Sciences.

  • Block C00084155.1a
  • Cervical Cancer, Invasive. Squamous cell carcinoma T1bN0M0 IB
  • Brightfield camera, Magnification: 20x
  • Sequencing instrument: Illumina NovaSeq 6000, flow cell HVFG7DSXY (lanes 1-4)
  • Sequencing depth: 73,046 reads per spot
  • Sequencing configuration: 28 bp read 1 (16 bp Visium spatial barcode, 12 bp UMI), 50 bp read 2 (transcript), 10 bp i7 sample barcode and 10 bp i5 sample barcode
  • Dual-Index set: SI-TS-E5
  • Slide: V10L13-019
  • Area: A1

Key metrics were:

  • Spots detected under tissue: 2,781
  • Median genes per spot: 7,276
  • Median UMI counts per spot: 26,050

This dataset is licensed under the Creative Commons Attribution license.