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Single Cell Gene Expression Flex

Important Workflow Update for Loading Concentration and Annealing TemperatureLearn more
product-intro
What is Single Cell Gene Expression Flex?

Single Cell Gene Expression Flex enables single cell gene expression on precious samples that were previously inaccessible due to logistical challenges in sample handling. Fixation at the point of sample collection preserves fragile biology and greatly streamlines workflows to provide critical insights on more samples.

Get startedFind User Guides

Documentation

We provide resources from protocols, user guides, and free-to-use analysis tools.

Experimental Design & Planning

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Gene Expression Flex.

Sample Prep

Prepare single cell/nuclei suspension from fresh samples for fixation or from archived samples by following our Demonstrated Protocols.

Probe Sets

Find a list of probes targeting protein-coding genes in human and mouse transcriptomes for the Single Cell Gene Expression Flex assay.

Library Construction

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Instruments

Refer to instrument-specific documents for seamless instrument operation.

Sequencing

Sequence your genome and exome library as per our provided recommendations.

Software Analysis

Analyze your genome and exome data using our pipelines and visualization tools.

Additional Applications

Protein
Reveal gene expression and protein expression from the same fixed cell.
Datasets

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

View datasets
Datasets

Videos

Chromium Next GEM Single Cell Gene Expression Flex video
How-to series
Chromium Next GEM Single Cell Gene Expression Flex
Chromium Next GEM Tissue Fixation and Dissociation video
Demonstrated Protocol series
Chromium Next GEM Tissue Fixation and Dissociation
Chromium Next GEM Single Cell Isolation from FFPE Sections video
Demonstrated Protocol series
Chromium Next GEM Single Cell Isolation from FFPE Sections
Q&A

Find answers to common technical questions, from sample prep through data analysis, about the Single Cell Gene Expression Flex workflow.

Q&A