10x Genomics Support/Single Cell Gene Expression Flex/Documentation/Library Construction/

Sample Index Sets for Fixed RNA Profiling

Each well of a dual index plate contains a mix of one unique i7 and one unique i5 sample index.

The files below contain the mappings from the sample index names to the associated set of Index (i7) and Index 2 (i5) oligonucleotides. Both the forward and reverse complement sequences are listed for the i5 sequence. Please see this article and our software support site for more information.

Please note that the sample index plate used for Fixed RNA Profiling Gene Expression libraries is different from the sample index plates used for Fixed RNA Profiling Feature Barcode libraries.

Fixed RNA Profiling Gene Expression libraries are made using the following sample index plate:

  • Dual Index Kit TS, Set A (PN-1000251): CSV | JSON

Fixed RNA Profiling Cell Surface Protein libraries (using TotalSeq™️-B) are made using the following sample index plate:

  • Dual Index Kit TT, Set A (PN-1000215): CSV | JSON

Fixed RNA Profiling Protein libraries (using Barcode Oligo Capture (TotalSeq™️-C)) are made using the following sample index plate:

  • Dual Index Kit TN, Set A (PN-1000250): CSV | JSON
Document Type
Specifications

Last Modified
September 13, 2023