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Visium HD Spatial Gene Expression

This assay requires CytAssist instrument Firmware v2.0.0 or higher. Refer to the Visium CytAssist with Readiness Test User Guide (CG000542) for details.
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What is Visium HD Spatial Gene Expression?

Visium HD Spatial Gene Expression is a single cell scale, high resolution, CytAssist-enabled, whole transcriptome probe-based assay compatible with mouse or human FFPE tissue sections placed on glass slides.

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Documentation

Find resources, from protocols to free-to-use analysis tools.

Experimental Planning and Design

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium HD Spatial Gene Expression protocols.

Tissue Prep & Staining

Best practices for handling FFPE tissue samples and generating tissue slides. Protocol outlines deparaffinization, staining, and decrosslinking of tissue sections.

Imaging

For imaging hardware, general image acquisition and analysis guidelines, and example images.

Probe Sets

Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium HD Spatial Gene Expression assay.

Library Construction

Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.

Instruments

Refer to instrument-specific documents for seamless instrument operation.

Sequencing

Sequence your Visium spatial library as per our provided recommendations.

Software Analysis

Analyze your Visium spatial data using our pipelines and visualization tools.

Datasets

Access free, publicly available data from a number of different sample types generated with Visium Spatial solutions

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Datasets

Videos - Visium CytAssist Instrument

Visium CytAssist Instrument video
How-to series
Visium CytAssist Instrument
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Q&A

Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.

Q&A