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HD 3’ Spatial Gene Expression
Visium Spatial

HD 3’ Spatial Gene Expression

This assay requires CytAssist instrument Firmware v2.0.0 or higher. Refer to the Visium CytAssist with Readiness Test User Guide (CG000542) for details.
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What is HD 3’ Spatial Gene Expression?

Single cell-scale resolution, unbiased, whole transcriptome, poly-A based gene expression assay compatible with Fresh Frozen tissue sections.

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Documentation

Find resources, from protocols to free-to-use analysis tools.

Experimental Planning and Design

Plan ahead by referring to the list of equipment, kits, and reagents needed for Visium HD 3’ Spatial Gene Expression protocols.

Tissue Prep & Staining

Best practices for preparing and handling Fresh Frozen tissue samples and generating tissue slides. Protocol outlines fixation, staining, and imaging of tissue sections.

Imaging

For imaging hardware, general image acquisition and analysis guidelines, and example images.

Library Construction

Construct a sequencing-ready Visium HD 3’ spatial library from Fresh Frozen tissue sections.

Instruments

Refer to instrument-specific documents for seamless instrument operation.

Sequencing

Sequence your Visium HD 3’ spatial library as per our provided recommendations.

Software Analysis

Analyze your Visium spatial data using our pipelines and visualization tools.

Datasets

Access free, publicly available data from a number of different sample types generated with Visium HD 3’ Spatial Gene Expression

View datasets
Datasets

Videos

Visium CytAssist Instrument video
How-to series
Visium CytAssist Instrument
Using the Visium CytAssist Alignment Aid, 6.5 mm video
How-to series
Using the Visium CytAssist Alignment Aid, 6.5 mm
Q&A

Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.

Q&A