Prepare optimal single cell/nuclei suspension by following our Demonstrated Protocols.

Sample Prep

Chromium Universal 3' Gene Expression provides single cell transcriptome 3' gene expression alongside the detection of surface protein expression or CRISPR edits in tens of thousands of cells. Scale experiments up or down with low-throughput and high-throughput solutions.

Measure single cell gene and cell surface protein expression simultaneously.

Single Cell Gene Expression with Cell Surface Protein Labeling

Cell Surface Protein

It is critical to choose the User Guide / Protocol that corresponds to your Reagent Kit version. Find the right User Guide via the button below.

Assess effects of CRISPR perturbations alongside single cell gene expression.

Single Cell Gene Expression with CRISPR Screening

CRISPR

Multiplex up to 12 samples per channel for increased sample throughput.

Single Cell Gene Expression with Cell Multiplexing technology

Cell Multiplexing

Profile 2,000–20,000 cells per channel of the Chromium Next GEM Chip M (2,000– 60,000 cells per channel with CellPlex).

Single Cell Gene Expression HT with Feature Barcode technology

High Throughput (HT)

Chromium Next GEM Single Cell 3' HT v3.1 - Dual Index

Seamless library construction capabilities on automated platforms.

3rd Party Automation

Additional Applications

Find resources from protocols to free-to-use analysis tools.

GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species

Universal 3'

Chromium X Series

Advanced system for multiomic analysis of hundreds to millions of cells.

Chromium

Gene Expression

3' gene expression profiling at scale with single cell resolution.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Gene Expression.

Experimental Design

Experimental Design & Planning

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Use pre-designed or custom panels for targeted gene expression, followed by sequencing and data analysis.

Targeted

Targeted Gene Expression (Optional)

Sequence your single cell library as per our provided recommendations.

Sequencing

Analyze your single cell data using our pipelines, visualization, and Cloud Analysis tools.

Software Analysis

Workflow Documentation

Epigenomic Profiling

Immune Profiling

Protein co-detection

Overview

User Guides

Datasets

Videos

Publications

Universal 3' Gene Expression

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Chromium Single Cell Universal 3' Gene Expression provides single cell transcriptome 3' gene expression alongside the detection of surface protein expression in tens of thousands of cells.

What is Universal 3' Gene Expression?

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

Explore how-to videos and training modules.

GEM-X: How to assemble, load and generate GEMs

GEM-X OCM: How to assemble, load and generate multiplex GEMs

Chromium Next GEM Single Cell 3' v3.1 - Dual Index

Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.