This Technical Note provides guidance on custom probe design, spike-in, and analysis for GEM-X Flex v2 applications.


GEM-Xテクノロジーを用いたプローブベースのアッセイで18,000以上のコーディング遺伝子をキャプチャー。プロジェクトのニーズに合わせてカスタマイズ可能

遺伝子発現Flex Apex

Chromium X シリーズ

Chromium

Advanced system for multiomic analysis of hundreds to millions of cells.

遺伝子発現Flex

Gene Expression

Epigenomic Profiling

Immune Profiling

Protein co-detection

Custom Probe Design for GEM-X Flex v2

Plan ahead by referring to the list of equipment, kits, and reagents needed for Flex Gene Expression.

Experimental Design

Experimental Design & Planning

Flex Gene Expression enables single cell gene expression on precious samples that were previously inaccessible due to logistical challenges in sample handling. Fixation at the point of sample collection preserves fragile biology and greatly streamlines workflows to provide critical insights on more samples.

Reveal gene expression and protein expression from the same fixed cell.

Flex with Protein Labeling

Protein

It is critical to choose the User Guide / Protocol that corresponds to your Reagent Kit version. Find the right User Guide via the button below.

Additional Applications

Measure single cell gene and cell surface protein expression simultaneously.

Cell Surface Protein

Find resources from protocols to free-to-use analysis tools.

Prepare single cell/nuclei suspension from fresh samples for fixation or from archived samples by following our Demonstrated Protocols.

Sample Prep

Find a list of probes targeting protein-coding genes for the Flex assay.

Probe Sets

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Sequence your single cell or nuclei library as per our provided recommendations.

Sequencing

Analyze your genome and exome data using our pipelines and visualization tools.

Software Analysis

Workflow Documentation

Overview

User Guides

Datasets

Videos

Flex Gene Expression

What is Flex Gene Expression?

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

GEM-X: How to assemble, load and generate GEMs

Chromium Next GEM Tissue Fixation and Dissociation

Chromium Next GEM Single Cell Isolation from FFPE Sections

Find answers to common technical questions, from sample prep through data analysis, about the Flex Gene Expression workflow.

Custom Probe Design for GEM-X Flex v2

Technical Note, CG000839

CG000839_GEM-X_Flex_v2_CustomProbeDesign_TechNote_Rev_B.pdf