The Xenium In Situ Gene and Protein Expression Solution enables simultaneous RNA and protein detection on the same human FFPE tissue section using the Xenium v1 assay chemistry, offering an integrated spatial multiomics approach. 27 immunology markers, organized into seven subpanels, enable robust and reliable identification of major immune cell types and subtypes. Xenium Explorer software facilitates the visualization and analysis of gene and protein expression data on a cell-to-cell basis. This Technical Note provides an overview of the Xenium Protein workflow and analysis, with data highlights.