List of human and mouse tissue types that were validated in-house with Xenium In Situ Gene Expression. FFPE tissues validated in-house were acquired from biobanks. Additional information about 10x Genomics pre-designed panels is available here.
Please note: sample preparation variability (such as fixation and embedding techniques), tissue biology, and slide handling may impact assay performance. In addition, disease state and presence of add-on panels may impact assay performance.
- For all sample types, except bone, refer to the Xenium In Situ for Fresh Frozen Tissues - Tissue Preparation Guide (CG000579) or Xenium In Situ for FFPE - Tissue Preparation Guide (CG000578), and additional best practices mentioned therein for optimal results.
- For human and mouse bone samples, it is crucial to follow the sample preparation guidance outlined in the Analysis of Bone Tissue using Xenium v1 In Situ Gene Expression Assay Technical Note (CG000816).
- All tissues listed below were processed per our standard documentation (CG000582).
Some tissues were tested with multiple tissue states. Select datasets can be found here.
Human tissue types
| Tissue-specific Pre-designed Panels | Tissue | Tissue States |
|---|---|---|
| Xenium Human Breast Gene Expression Panel | Breast | Infiltrating Ductal Carcinoma, Invasive Lobular Carcinoma, Angiocarcinoma, Other cancer |
| Xenium Human Brain Gene Expression Panel | Brain | Non-diseased, Alzheimer's Disease, Glioblastoma Multiforme |
| Xenium Human Lung Gene Expression Panel | Lung | Non-diseased, Invasive Adenocarcinoma, Idiopathic Pulmonary Fibrosis |
| Xenium Human Skin Gene Expression Panel | Skin | Melanoma |
| Xenium Human Colon Gene Expression Panel | Colon | Adenocarcinoma |
The Xenium Human Multi-Tissue and Cancer Panel has been validated for several tissues and tissue states:
| Tissue | Tissue States |
|---|---|
| Breast | Invasive Ductal Carcinoma |
| Bone | Non-diseased Femur Bone, Non-diseased Bone Marrow, Acute Lymphoid Leukemia Bone Marrow |
| Colon | Non-diseased, Adenocarcinoma, Diseased |
| Eye | Non-diseased |
| Heart | Non-diseased, Leiomyosarcoma |
| Kidney | Non-diseased, Papillary Renal Cell Carcinoma, Diseased |
| Large Intestine | Diseased |
| Liver | Non-diseased, Fibrosis, Diseased |
| Lung | Non-diseased, Non-small Cell Lung Carcinoma, Fibrosis |
| Lymph Node | Non-diseased, Lymphofollicular Hyperplasia, Diseased |
| Pancreas | Non-diseased, Adenocarcinoma, Diseased |
| Skin | Non-diseased, Melanoma, Diseased |
| Tonsil | Non-diseased |
The Xenium Human Immuno-Oncology Profiling Panel has been validated for several tissues and tissue states:
| Tissue | Tissue States |
|---|---|
| Brain | Glioblastoma |
| Breast | Non-diseased, Ductal Carcinoma In Situ, Invasive Ductal Carcinoma |
| Colon | Adenocarcinoma |
| Kidney | Renal Cell Carcinoma |
| Liver | Hepatocellular Carcinoma |
| Lung | Non-small Cell Lung Carcinoma |
| Lymph Node | Lymphoma |
| Ovary | Serous Adenocarcinoma |
| Pancreas | Ductal Adenocarcinoma |
| Prostate | Acinar Adenocarcinoma |
| Skin | Melanoma |
Mouse tissue types
| Pre-designed Panel | Tissue | Tissue State |
|---|---|---|
| Xenium Mouse Brain Gene Expression Panel | Brain | Non-diseased, Wild type & Trangenic mouse model of Alzheimer's Disease |
| Xenium Mouse Tissue Atlassing Panel | Adipose Tissue | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Bone | Non-diseased Femur |
| Xenium Mouse Tissue Atlassing Panel | Brain | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Colon | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Heart | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Kidney | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Liver | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Lung | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Skin | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Spleen | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Thymus | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Whole body, Embryonic day 17.5 | Non-diseased |
| Xenium Mouse Tissue Atlassing Panel | Whole body, Postnatal day zero | Non-diseased |