Protocols labeled "2.0" are compatible with Visium HD 6.5 mm and 11 mm slides and contain a "Featured" tag. For ongoing Visium HD 6.5 mm projects, please use original protocol versions.
This document provides guidance on preparation of Fresh Frozen tissue blocks and generating tissue slides. Demonstrated protocols for performing tissue staining (H&E and IF) are included.
Key companion documents for use with this document:
- Visium HD Spatial Gene Expression Protocol Planner (CG000698)
- Tissue Slide Alignment Instruction, Quick Reference Cards (CG000548)
- Visium S3 for Tissue Slide Cassette Assembly Quick Reference Cards (CG000730)
For use with:
- Visium HD, Human Transcriptome, 6.5 mm, PN-1000675 (4 rxns) / PN-1000673 (16 rxns)
- Visium HD, Mouse Transcriptome, 6.5 mm, PN -1000676 (4 rxns) / PN-1000674 (16 rxns)
- Visium CytAssist Reagent Accessory Kit, PN-1000499
- Dual Index Kit TS Set A, 96 rxns, PN-1000251