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Single Cell Gene Expression HT Demonstrated Protocol Compatibility Table

Single Cell Gene Expression HT Demonstrated Protocol Compatibility Table

ProtocolHTHT with CRISPR ScreeningHT with Cell Surface ProteinHT with Cell MultiplexingHT with CRISPR Screening & Cell MultiplexingHT with Cell Surface Protein & Cell Multiplexing
Single Cell Protocols - Cell Preparation Guide✓✓✓✓✓✓
Methanol Fixation of Cells for Single Cell RNA Sequencing-✘✘✘✘✘
Nuclei Isolation from Cell Suspensions & Tissues for Single Cell RNA Sequencing✓*✘✘✓✘✘
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling✓*-✓*✓*-✓*
Tumor Dissociation for Single Cell RNA Sequencing✓-✓✓-✓
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing✓✓✓✓✓✓
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing✓*✓*✓*✓*✓✓*
Moss Protoplast Suspension for Single Cell RNA Sequencing------
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing✓✓✓✓✓✓
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing✓-✓✓-✓
Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing✓-✓✓-✓
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols✓-✓--✓
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing✓*✓*✓*✓*✓*✓*
Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols---✓✓✓
High Throughput Sample Preparation for Single Cell RNA Sequencing---✓--

✓ Compatible (tested in-house) ✓* Untested but low risk ** Compatible but unsupported - Untested, use at your own risk ✘ Incompatible

Document Type
Demonstrated Protocol
Last Modified
June 17, 2022