10x Genomics Support/Visium HD Spatial Gene Expression/Documentation/Tissue Prep & Staining/

Visium HD Spatial Gene Expression Tested Tissues

Tested Tissue List

List of tissue types that were tested by 10x and found to perform well with the Visium HD Spatial Gene Expression assay. Tissues tested in-house were acquired from biobanks. Please note: sample preparation variability (such as fixation and embedding techniques), tissue biology, and slide handling may impact assay performance. Tissues listed below were stained as per our documentation. Visium HD datasets can be found here.

SpeciesTissuePrimary diagnosisImportant considerations
HumanBrainHealthy
HumanBrainCancer, Glioblastoma
HumanBreastHealthyTissue type is rich in connective tissue, we recommend utilizing Schott Nexterion Slide H (Schott, PN-1800434) to minimize risk of tissue detachment.
HumanBreastCancer, Ductal Carcinoma in SituTissue type is rich in connective tissue, we recommend utilizing Schott Nexterion Slide H (Schott, PN-1800434) to minimize risk of tissue detachment.
HumanColonHealthyAmount of connective tissue can vary with this tissue type. For tissue blocks rich in connective tissue, they may benefit from utilizing Schott Nexterion Slide H (Schott, PN-1800434) to minimize risk of tissue detachment.
HumanColonCancer, AdenocarcinomaAmount of connective tissue can vary with this tissue type. For tissue blocks rich in connective tissue, they may benefit from utilizing Schott Nexterion Slide H (Schott, PN-1800434) to minimize risk of tissue detachment.
HumanKidneyHealthy
HumanLiverCancer, Hepatocellular carcinoma
HumanLungCancer, Adenocarcinoma
HumanLymph NodeHealthy
HumanOvaryCancer, Serous Carcinoma
HumanPancreasHealthy
HumanProstateCancer, Adenocarcinoma
HumanSkinCancer, Melanoma
HumanSpleenHealthyTissue type commonly contains regions rich in red blood cells which may result in a reduction in usable reads in these localized area(s) indicated by an increase in split-mapped reads on the web summary. These wasted reads will be filtered out of the final data.
HumanTonsilChronic tonsilitis
Human (TMA)Brain, Breast, Colon, Lung, Skin, Spleen
(3 x 3 Array, 1.5 mm Diameter, .5 mm Edge-to-Edge Distance)
Healthy & DiseasedExpertise required for generating TMA blocks, sectioning and tissue placement. If array is composed of tissue cores rich in connective tissue or prone to detachment, we recommend utilizing Schott Nexterion Slide H (Schott, PN-1800434).
MouseBrainHealthy
MouseEmbryo E17.5, E15.5Healthy
MouseHeartHealthy
MouseIntestine (Swiss roll)Healthy
MouseKidneyHealthy
MouseLungHealthy
Document Type
Specifications

Last Modified
January 23, 2024