Peripheral blood mononuclear cells (PBMCs) from a healthy donor were obtained by 10x Genomics from AllCells and were labelled with a panel of 17 TotalSeq™-B antibodies (BioLegend). The same cells were used to generate Chromium Single Cell 3’ (Dual Index) data.
Libraries were prepared following the Chromium Single Cell 3' Reagent Kits v3.1 User Guide with Feature Barcode technology for Cell Surface Protein (CG000206) and sequenced on Illumina NovaSeq 6000.
Single Cell 3’ Cell Surface Protein v3.1 Single Index Library
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Sequencing Depth: 54,962 read pairs per cell
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Paired-end, single indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i7 index: 8 cycles (sample index) Read 2: 91 cycles (transcript)
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Analysis Parameters Used: Run with --expect-cells=1,000
Key metrics were:
- Cell detected: 1,206
- Median UMI counts per cell: 6,233
- Median genes per cell: 1,836