10k Human PBMCs Stained with TotalSeq™-C Human TBNK Cocktail, Chromium GEM-X Single Cell 5'
Single Cell Immune Profiling dataset analyzed using Cell Ranger 8.0.0
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Peripheral blood mononuclear cells (PBMCs) from a healthy human male donor, aged 18-35, were obtained by 10x Genomics from AllCells.
2 million cells were stained with TotalSeq™-C Human TBNK Cocktail (BioLegend, Cat# 399903) following the manufacturer's guidance for antibody reconstitution and the 10x demonstrated protocol for Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols with Feature Barcode technology (CG000149, Rev D). After staining, cells were washed using the Wash Protocol 1 option.
Gene Expression, VDJ, and Cell Surface Protein libraries were generated as described in the Chromium GEM-X Single Cell 5' Reagent Kits v3 with Feature Barcode technology for Cell Surface Protein & Immune Receptor Mapping User Guide (CG000734) and sequenced on an Illumina NovaSeq 6000 with approximately 62,000 read pairs per cell.
Paired-end, dual indexing:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.
This dataset is licensed under the Creative Commons Attribution license.