All datasets

20k Human PBMCs, 3' HT v3.1, Chromium X

Universal 3' dataset analyzed using Cell Ranger 6.1.0

Number of samples on this datasets page1

Human peripheral blood mononuclear cells (PBMCs) of a healthy female donor aged 25-30 were obtained by 10x Genomics from AllCells.

Libraries were generated from ~33,000 cells (23,837 cells recovered) as described in the Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 User Guide (CG000416 Rev A) using the Chromium X and sequenced on an Illumina NovaSeq 6000 to a read depth of appriximately 35,000 mean reads per cell.

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript).

Run with --expect-cells=20000.

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Additional Applications

High Throughput

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium X Series

Species

Human

Anatomical Entity

Blood

Disease State

healthy

Cells or Nuclei

Cells

Selected Cell Types

PBMCs

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

23,837

Donor Count

1

Date Published

2021-08-09