5k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells (v3 chemistry)

Single Cell Gene Expression dataset analyzed using Cell Ranger 3.0.0

Assess data quality

View summary metrics to assess data quality and more.

Visualize and explore data

Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software.

Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior (the same cells were used to generate hgmm_1k_v3, hgmm_1k_v2, hgmm_10k_v3).

5,366 cells detected
Sequenced on Illumina NovaSeq with approximately 58,000 reads per cell
28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
run with --expect-cells=5000

This dataset is licensed under the Creative Commons Attribution license.