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5k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells (v3 chemistry)

Single Cell Gene Expression dataset analyzed using Cell Ranger 3.0.0

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1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior (the same cells were used to generate hgmm_1k_v3, hgmm_1k_v2, hgmm_10k_v3).

  • 5,366 cells detected
  • Sequenced on Illumina NovaSeq with approximately 58,000 reads per cell
  • 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=5000

This dataset is licensed under the Creative Commons Attribution license.