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500 Peripheral Blood Mononuclear Cells (PBMCs) from a Healthy Donor (Next GEM v1.1)

Single Cell ATAC dataset analyzed using Cell Ranger ATAC 1.1.0

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Peripheral blood mononuclear cells (PBMCs) from a healthy donor.

Libraries were prepared following the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 (User Guide CG000209 Rev A). All PBMC libraries (v1.1 chemistry vs. v1 chemistry at all cell loads) were prepared with the same nuclei preparation.

  • ~750 transposed nuclei were loaded. ~500 expected nuclei recovered. 623 nuclei were detected.
  • Sequenced on Illumina NovaSeq with approximately 54k read pairs per cell.
    • 50 bp Read 1
    • 8 bp i7 index (sample index)
    • 16 bp i5 (10x Barcode)
    • 49 bp Read 2

This dataset is licensed under the Creative Commons Attribution license.