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5k Human Melanoma Tumor Isolated with Chromium Nuclei Isolation Kit

Single Cell Immune Profiling dataset analyzed using Cell Ranger 7.0.0

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Nuclei were isolated from ~25 mg of fresh frozen human melanoma purchased from Discovery Life Sciences following the Chromium Nuclei Isolation Reagent Kits Sample Prep User Guide (CG000505).

Gene Expression libraries were prepared following the Chromium Single Cell 5'v2 Reagent Kits User Guide (CG000331) and targeting 5,000 cells. Libraries were sequenced on an Illumina NovaSeq instrument targeting 20,000 reads per cell.

Sequencing data were processed using Cell Ranger, with web_summary.html and output files available below.

  • Sequencing instrument: Illumina NovaSeq 6000
  • Sequencing depth: 86,324 mean reads per cell
  • Sequencing configuration: Paired-end, dual indexing;
    • Read 1: 28 cycles (16 bp barcode, 12 bp UMI)
    • i5 index: 10 cycles (sample index)
    • i7 index: 10 cycles (sample index)
    • Read 2: 90 cycles (transcript)
  • Cells detected: 5,700
  • Median genes per cell: 1,220
  • Median UMIs per cell: 1,885

This dataset is licensed under the Creative Commons Attribution license.