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Human Ovarian Cancer (FFPE)

Spatial Gene Expression dataset analyzed using Space Ranger 1.3.0

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5 µm section from serous papillary carcinoma of human ovarian. FFPE tissue purchased from iSpecimen.

  • Block 108906
  • Ovarian Cancer, Serous papillary carcinoma
  • Brightfield camera, Magnification: 20x
  • Sequencing instrument: Illumina NovaSeq 6000, flow cell HCCVHDSX2 (lanes 1-4)
  • Sequencing depth: 63,836 reads per spot
  • Sequencing configuration: 28 bp read 1 (16 bp Visium spatial barcode, 12 bp UMI), 75 bp read 2 (transcript), 10 bp i7 sample barcode and 10 bp i5 sample barcode
  • Dual-Index set: SI-TS-H1
  • Slide: V10L13-020
  • Area: D1

Key metrics were:

  • Spots detected under tissue: 3,455
  • Median genes per spot: 7,621
  • Median UMI counts per spot: 28,927

This dataset is licensed under the Creative Commons Attribution license.