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Integrated GEX, TotalSeq™-C, and BCR Analysis of Chromium Connect Generated Library from 7k Melanoma Skin Samples

Single Cell Immune Profiling dataset analyzed using Cell Ranger 6.1.2

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Melanoma samples were isolated from skin (lot BTC1000-E1110036711041816MS). Gene Expression, BCR, and Antibody Capture libraries were generated from a single Chromium Connect channel. Antibody libraries were generated using a BioLegend TotalSeq™-C TBNK panel. The BCR library was downsampled to 5,000 reads per cell. Libraries were prepared following the Chromium Next GEM Automated Single Cell 5' Reagent Kits v2 User Guide (CG0000507).

  • 7,000 cells targeted
  • 5,925 cells detected
  • 66,247 mean reads per cell
  • Sequenced on Illumina NovaSeq with 28 bp read 1, 90 bp read 2, 10 bp i5 sample barcode, and 10 bp i7 sample barcode
  • Run with Cell Ranger 6.1.2

This dataset is licensed under the Creative Commons Attribution license.