Human peripheral blood mononuclear cells (PBMCs) of a healthy female donor aged 25 were obtained by 10x Genomics from AllCells. Whole transcriptome libraries were generated with Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) User Guide (CG000315).
The targeted gene expression libraries were generated with Targeted Gene Expression Reagent Kits User Guide (CG000293) using the Human Immunology Panel (PN-1000246) and sequenced on an Illumina NovaSeq 6000.
Targeted Gene Expression Library
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Sequencing Depth: 13,211 reads per cell
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Paired-end (28 X 90), dual-indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i7 index: 10 cycles i5 index: 10 cycles Read 2: 90 cycles (transcript)
The key metrics were:
- Cells detected: 10,497
- Reads Mapped Confidently to Targeted Transcriptome: 87.5%
- Total targeted genes detected: 982