免疫学

重新定义您对先天免疫
和适应性免疫的理解

揭示免疫细胞的复杂性

解决免疫系统的错综复杂之处，有助于在传染病、自身免疫性疾病、免疫疗法开发和癌症研究中获得切实可行的深入见解。了解如何以前所未有的广度、深度和精度来探索免疫学，并使用10x Genomics技术真正改变人类健康的进程。

 
Immunology Brochure

不只停留在表面

为了深入了解协调配合的先天性和适应性免疫应答，研究人员需要能够在细胞和分子分辨率下对组织进行特征分析的工具。单细胞测序技术就是这样一种方法，从而识别对病原体免疫应答的分子机制，追踪涉及的细胞类型和状态，并发现用于免疫疗法的新生物标记。

下载全面的《免疫学研究指南》，了解单细胞测序技术如何解决免疫学相关重大问题的更多信息。

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特色网络研讨会 & 研讨会

由免疫学专家带我们全面了解免疫学

揭示潜在的适应性和先天免疫反应机制，以真正改变人类健康的进程。通过这些免疫学主题的网络研讨会，进一步了解免疫学家和10x Genomics的科学家如何通过现有的单细胞解决方案得到相关的见解和发现。

High-Throughput B Cell Screening for Immune Profiling and Antibody Discovery in HIV

Speaker: Ian Setliff, laboratory of Ivelin Georgiev, Vanderbilt University

Learn how researchers from Vanderbilt University used single cell immune sequencing to identify a new panel of broadly neutralizing antibodies against HIV-infected B cells.

 
Constructing the Human Immune Census

Speaker: Jimmie Ye, UCSF

Listen to UCSF Assistant Professor Jimmie Ye describe his team’s efforts to build a molecular survey of gene expression in one million immune cells from 200 different blood samples, including samples from patients with lupus.

 
Redefining Immune Cell Phenotyping & Characterization

Speaker: Sarah Taylor, Daniel Riorden, Dagmar Walter

Discover the power of multiomic approaches for phenotyping immune cells as 10x Genomics scientists explain how they used the Chromium Single Cell Immune Profiling Solution to characterize gene expression, T- and B-cell clonotypes and cell surface markers in PBMC samples from a cytomegalovirus (CMV) seropositive and seronegative donor.

 
A New Way of Exploring Immunity

Speaker: Mike Stubbington, Alvaro Martinez Barrio, Dagmar Walter

Explore the capabilities of single cell immune sequencing solutions to characterize the adaptive immune system as 10x Genomics scientists explain how to uncover gene and cell surface protein expression, TCR-pMHC binding specificity, and paired alpha/beta T-cell receptor (TCR) sequences in 160,914 single CD8+ T cells, including 62,858 antigen binding cells.

免疫学研究摘要

《客户研究摘要》将10x Genomics的客户出版物精简为易于理解的实验摘要，详细介绍了如何进行实验，分析数据并得出结论。我们不断增加的摘要列表展示了免疫学家如何使用10x Genomics产品来了解免疫应答背后的多种细胞和分子机制。

Single-Cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment

Azizi et al., Cell 2018

Performed single cell transcriptional and immune profiling of breast carcinoma-resident immune cells, showing a continuum of activation and differentiation states for tumor-associated T cells and macrophages.

Gene Expression Variability Across Cells and Species Shapes Innate Immunity

Hagai et al., Nature 2018

Used single cell and bulk RNA sequencing to chart the evolution of the innate immune response in mammals, identifying genes that were differentially expressed upon immune stimulation.

Single Cell Analysis of Fate-mapped Macrophages Reveals Heterogeneity During Atherosclerosis Progression and Regression

Lin et al., JCI Insight 2019

Used single cell RNA sequencing to characterize the cellular states of macrophages in atherosclerotic plaques, showing a spectrum of macrophage activation states.

B细胞受体序列与抗原特异性的高通量映射

Setliff et al., Cell 2019

开发出一种称为LIBRA-seq的方法，可将B细胞受体（BCR）成对的重链和轻链序列快速映射到其相应抗原。

特色资源

捕获免疫系统的整体快照

以您希望的方式灵活开展免疫学研究。如今可通过单细胞和多组学方法对免疫应答和免疫细胞进行全面表征，包括同时检测细胞表面蛋白、抗原特异性，以及免疫组库、基因表达和染色质分析。通过将10x Genomics技术整合到现有的流式细胞仪工作流程中，您能够对成千上万个免疫细胞开展复杂的表型分析。

了解我们最新的演示实验，用Feature Barcoding技术强大的可扩展性生成丰富的数据集，以阐明TCR-pMHC的交互机制

了解如何对单个T细胞开展多组学表型分析，包括细胞表面蛋白和抗原特异性表征。

了解如何鉴定各个细胞之间的调控元件差异，从而更好地鉴定免疫细胞类型。

 

观看AGBT 2019的演示文稿，了解如何利用多组学方法来加深对适应性免疫的理解。

精选文献

进入新层次的表征和分辨率

10x Genomics的解决方案使得免疫学研究人员能够跳出传统的细胞分型方法，更全面地了解先天性和适应性免疫。了解研究人员如何将10x Genomics的技术整合到现有的工作流程中。 下载免疫学研究集锦

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High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity
I Setliff et al. Cell. 2019
Performed high-throughput interrogation of antibody-antigen interactions using single cell sequencing to screen B cells from human HIV-infection samples against large libraries of viral antigens for antibody discovery.
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Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion
Satpathy AT et al. Nat Biotechnol. 2019
Analyzed serial tumor biopsies before and after PD-1 blockade, revealing chromatin regulators of therapy-responsive T cell subsets and identified a shared regulatory program that governs intratumoral CD8+ T cell exhaustion and CD4+ T follicular helper cell development.
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The bone marrow microenvironment at single-cell resolution
Tikhonova AN et al. Nature. 2019
Mapped the transcriptional landscape of mouse bone marrow microenvironment both at homeostasis and under conditions of stress-induced haematopoiesis revealing previously unappreciated levels of cellular heterogeneity and considerable transcriptional remodelling under stress conditions
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Memory CD4+ T cells are generated in the human fetal intestine
Li N et al. Nat Immunol. 2019
Characterize the CD4+ T cell compartment in the human fetal intestine and found evidence for the generation of memory-like CD4+ T cells in the human fetal intestine that is consistent with exposure to foreign antigens.

我们的解决方案

深入了解我们的解决方案如何以前所未有的深度、广度和精度探索免疫系统

Chromium单细胞免疫分析解决方案让您能够同时分析数百至数万个人或小鼠T细胞和B细胞中的适应性免疫反应的细胞背景和免疫组库。

了解更多

Chromium单细胞ATAC解决方案让您能够发现表观遗传变化带来的细胞异质性，并更好地了解基因调控，从而更好地定义细胞状态和类型，便于谱系追踪和生物标志物发现。

了解更多

Chromium单细胞基因表达解决方案为揭开细胞间的基因表达差异和鉴定复杂生物样品中的稀有细胞提供了一种前所未有的方法。

了解更多

Visium空间基因表达解决方案是新一代分子成像技术，可以让您基于总mRNA进行全局性的组织分类，或专注于任何感兴趣的区域，以发现新的基因表达谱。

了解更多

利用10x Genomics解决方案，重新
定义您对先天性和适应性免疫的认识