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5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor with cell surface proteins (v3 chemistry)

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor with cell surface proteins (Next GEM)

Cell Ranger 3.0.2

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v3 chemistry)

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM)

1k 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells (v3 chemistry)

1k 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells (Next GEM)

5k 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells (Next GEM)

10k 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells (Next GEM)

5k Cells from a combined cortex, hippocampus and subventricular zone of an E18 mouse (v3 chemistry)

5k Cells from a combined cortex, hippocampus and subventricular zone of an E18 mouse (Next GEM)

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor with cell surface proteins (v3 chemistry)

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor with cell surface proteins (Next GEM)

Chromium Demonstration (v3 Chemistry)

Cell Ranger 3.0.0

1k PBMCs from a Healthy Donor – Gene Expression and Cell Surface Protein

10k PBMCs from a Healthy Donor – Gene Expression and Cell Surface Protein

10k Cells from a MALT Tumor – Gene Expression and Cell Surface Protein

1k PBMCs from a Healthy Donor (v2 chemistry)

1k PBMCs from a Healthy Donor (v3 chemistry)

10k PBMCs from a Healthy Donor (v3 chemistry)

1k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells (v2 chemistry)

1k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells (v3 chemistry)

5k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells (v3 chemistry)

10k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells (v3 chemistry)

1k Brain Cells from an E18 Mouse (v2 chemistry)

1k Brain Cells from an E18 Mouse (v3 chemistry)

10k Brain Cells from an E18 Mouse (v3 chemistry)

1k Heart Cells from an E18 mouse (v2 chemistry)

1k Heart Cells from an E18 mouse (v3 chemistry)

10k Heart Cells from an E18 mouse (v3 chemistry)

Chromium Demonstration (v2 Chemistry)

Cell Ranger 2.1.0

100 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

1k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

6k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

12k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

4k PBMCs from a Healthy Donor

8k PBMCs from a Healthy Donor

9k Brain Cells from an E18 Mouse

1k Brain Cells from an E18 Mouse

1k Brain Nuclei from an E18 Mouse

2k Brain Nuclei from an Adult Mouse (>8 weeks)

2k Brain Cells from an E18 Mouse

2k Fixed Brain Cells from an E18 Mouse

2k Fixed (for 6 days at -80ºC) Brain Cells from an E18 Mouse

3k Pan T Cells from a Healthy Donor

4k Pan T Cells from a Healthy Donor

Aggregate of t_3k and t_4k

Cell Ranger 2.0.1

100 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

1k Brain Cells from an E18 Mouse

2k Brain Nuclei from an Adult Mouse (>8 weeks)

1k Brain Nuclei from an E18 Mouse

1k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

6k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

12k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

4k PBMCs from a Healthy Donor

8k PBMCs from a Healthy Donor

9k Brain Cells from an E18 Mouse

3k Pan T Cells from a Healthy Donor

4k Pan T Cells from a Healthy Donor

Aggregate of t_3k and t_4k

Cell Ranger 1.3.0

100 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

1k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

6k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

12k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

4k PBMCs from a Healthy Donor

8k PBMCs from a Healthy Donor

9k Brain Cells from an E18 Mouse

3k Pan T Cells from a Healthy Donor

4k Pan T Cells from a Healthy Donor

Aggregate of t_3k and t_4k

Cell Ranger 1.2.0

100 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

1k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

6k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

12k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

4k PBMCs from a Healthy Donor

8k PBMCs from a Healthy Donor

9k Brain Cells from an E18 Mouse

3k Pan T Cells from a Healthy Donor

4k Pan T Cells from a Healthy Donor

1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

33k PBMCs from a Healthy Donor

6k PBMCs from a Healthy Donor

3k PBMCs from a Healthy Donor

Cell Ranger 1.0.0

1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

3k PBMCs from a Healthy Donor

Single Cell 3′ Paper: Zheng et al. 2017

Cell Ranger 1.1.0

293T and 3T3 Cell Mixture

50%:50% Donor B: Donor C PBMC Mixture

AML035 Pre-transplant BMMCs

AML035 Post-transplant BMMCs

CD14+ Monocytes

Fresh 68k PBMCs (Donor A)

99%:1% Jurkat:293T Cell Mixture

90%:10% Donor B: Donor C PBMC Mixture

ERCC (1k GEMS, 1:10 Dilution)

Frozen PBMCs (Donor B)

CD34+ Cells

293T Cells

Jurkat Cells

CD56+ Natural Killer Cells

AML027 Pre-transplant BMMCs

CD8+ Cytotoxic T cells

CD4+ Helper T Cells

CD4+/CD25+ Regulatory T Cells

Frozen PBMCs (Donor A)

AML027 Post-transplant BMMCs

CD19+ B Cells

Frozen BMMCs (Healthy Control 2)

50%:50% Jurkat:293T Cell Mixture

CD8+/CD45RA+ Naive Cytotoxic T Cells

Frozen BMMCs (Healthy Control 1)

Frozen PBMCs (Donor C)

CD4+/CD45RA+/CD25- Naive T cells

CD4+/CD45RO+ Memory T Cells

99%:1% Donor B: Donor C PBMC Mixture

单细胞免疫分析数据集

V(D)J + 5′ Gene Expression Next GEM Demonstration (v1.1 Chemistry)

Cell Ranger 3.1.0

Human PBMC – 5′ Cell Surface Protein-Only (v1)

Human PBMC – 5′ Cell Surface Protein-Only (Next GEM v1.1)

PBMCs of a Healthy Donor (v1)

PBMCs of a Healthy Donor (Next GEM v1.1)

PBMCs from C57BL/6 mice (v1)

PBMCs from C57BL/6 mice (Next GEM v1.1)

PBMCs of a Healthy Donor (v1, 150×150)

PBMCs of a Healthy Donor (Next GEM v1.1, 150×150)

PBMCs from C57BL/6 mice (v1, 150×150)

PBMCs from C57BL/6 mice (Next GEM v1.1, 150×150)

Cell Ranger 3.0.2

PBMCs of a Healthy Donor (v1)

PBMCs of a Healthy Donor (Next GEM v1.1)

PBMCs from C57BL/6 mice (v1)

PBMCs from C57BL/6 mice (Next GEM v1.1)

Application Note – A New Way of Exploring Immunity

Cell Ranger 3.0.2

CD8+ T cells of Healthy Donor 1

CD8+ T cells of Healthy Donor 2

CD8+ T cells of Healthy Donor 3

CD8+ T cells of Healthy Donor 4

Application Note – Redefining Cellular Phenotyping

Cell Ranger 3.0.2

Sorted PBMCs from a CMV seropositive donor – 5′ gene expression and cell surface protein

Sorted PBMCs from a CMV seropositive donor – TCR enrichment from amplified cDNA

Unsorted PBMCs from a CMV seropositive donor – 5′ gene expression and cell surface protein

Unsorted PBMCs from a CMV seropositive donor – TCR enrichment from amplified cDNA

Aggregated PBMCs from a CMV seropositive donor – 5′ gene expression and cell surface protein

Sorted PBMCs from a CMV seronegative donor – 5′ gene expression and cell surface protein

Sorted PBMCs from a CMV seronegative donor – TCR enrichment from amplified cDNA

Unsorted PBMCs from a CMV seronegative donor – 5′ gene expression and cell surface protein

Unsorted PBMCs from a CMV seronegative donor – TCR enrichment from amplified cDNA

Aggregated PBMCs from a CMV seronegative donor – 5′ gene expression and cell surface protein

V(D)J + 5′ Gene Expression + Cell Surface Protein – Human

Cell Ranger 3.0.0

PBMCs of a healthy donor – 5′ gene expression and cell surface protein

PBMCs of a healthy donor – TCR enrichment from amplified cDNA

PBMCs of a healthy donor – Ig enrichment from amplified cDNA

V(D)J + 5′ Gene Expression – Mouse

Cell Ranger 3.0.0

PBMCs from C57BL/6 mice – 5’ gene expression

PBMCs from C57BL/6 mice – TCR enrichment from amplified cDNA

PBMCs from C57BL/6 mice – Ig enrichment from amplified cDNA

PBMCs from BALB/c mice – 5’ gene expression

PBMCs from BALB/c mice – TCR enrichment from amplified cDNA

PBMCs from BALB/c mice – Ig enrichment from amplified cDNA

Cell Ranger 2.2.0

PBMCs from C57BL/6 mice – 5’ gene expression

PBMCs from C57BL/6 mice – TCR enrichment from amplified cDNA

PBMCs from C57BL/6 mice – Ig enrichment from amplified cDNA

PBMCs from BALB/c mice – 5’ gene expression

PBMCs from BALB/c mice – TCR enrichment from amplified cDNA

PBMCs from BALB/c mice – Ig enrichment from amplified cDNA

Direct TCR Enrichment – Mouse

Cell Ranger 2.2.0

C57BL/6 mouse splenocytes – Direct TCR enrichment

Direct Ig Enrichment – Mouse

Cell Ranger 2.2.0

B cells isolated from C57BL/6 mouse splenocytes – Direct Ig enrichment

V(D)J + 5′ Gene Expression – Human

Cell Ranger 2.2.0

NSCLC tumor – 5’ gene expression

NSCLC tumor – TCR enrichment from amplified cDNA

NSCLC tumor – Ig enrichment from amplified cDNA

PBMCs of a healthy donor – 5′ gene expression

PBMCs of a healthy donor – TCR enrichment from amplified cDNA

PBMCs of a healthy donor – Ig enrichment from amplified cDNA

Direct TCR Enrichment – Human

Cell Ranger 2.2.0

Pan T cells isolated from PBMCs of a healthy donor – Direct TCR enrichment

CD8+ T cells isolated from PBMCs of a healthy donor – Direct TCR enrichment

CD4+ T cells isolated from PBMCs of a healthy donor – Direct TCR enrichment

Jurkat, clone E6-1 – Direct TCR enrichment

Direct Ig Enrichment – Human

Cell Ranger 2.2.0

CD19+ B cells isolated from PBMCs of a healthy donor – Direct Ig enrichment

GM12878 cell line – Direct Ig enrichment

Loupe V(D)J Browser Tutorial Dataset – Human

Cell Ranger 2.1.0

CD8+ Cytotoxic T cells from mononuclear cells of a healthy donor

单细胞ATAC数据集

Chromium Next GEM ATAC Demonstration (v1.1 Chemistry)

Cell Ranger ATAC 1.2.0

500 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (v1.0)

500 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (Next GEM v1.1)

1k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (v1.0)

1k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (Next GEM v1.1)

5k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (v1.0)

5k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (Next GEM v1.1)

10k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (v1.0)

10k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (Next GEM v1.1)

500 Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v1.0)

500 Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM v1.1)

1k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v1.0)

1k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM v1.1)

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v1.0)

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM v1.1)

10k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v1.0)

10k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM v1.1)

Cell Ranger ATAC 1.1.0

500 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (v1.0)

500 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (Next GEM v1.1)

1k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (v1.0)

1k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (Next GEM v1.1)

5k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (v1.0)

5k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (Next GEM v1.1)

10k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (v1.0)

10k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells (Next GEM v1.1)

500 Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v1.0)

500 Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM v1.1)

1k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v1.0)

1k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM v1.1)

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v1.0)

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM v1.1)

10k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (v1.0)

10k Peripheral blood mononuclear cells (PBMCs) from a healthy donor (Next GEM v1.1)

Single Cell ATAC Demonstration

Cell Ranger ATAC 1.2.0

500 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells

1k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells

5k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells

10k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor

10k Peripheral blood mononuclear cells (PBMCs) from a healthy donor

Fresh cortex, hippocampus, and ventricular zone from embryonic mouse brain (E18)

Dissociated and cryopreserved cortex, hippocampus, and ventricular zone cells from embryonic mouse brain (E18)

Flash frozen cortex, hippocampus, and ventricular zone from embryonic mouse brain (E18)

Fresh cortex from adult mouse brain (P50)

Cell Ranger ATAC 1.1.0

500 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells

1k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells

5k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells

10k 1:1 mixture of fresh frozen human (GM12878) and mouse (A20) cells

5k Peripheral blood mononuclear cells (PBMCs) from a healthy donor

10k Peripheral blood mononuclear cells (PBMCs) from a healthy donor

Fresh cortex, hippocampus, and ventricular zone from embryonic mouse brain (E18)

Dissociated and cryopreserved cortex, hippocampus, and ventricular zone cells from embryonic mouse brain (E18)

Flash frozen cortex, hippocampus, and ventricular zone from embryonic mouse brain (E18)