GEM-X Flex Gene Expression Kit Compatibility Table

Plan ahead by referring to the list of equipment, kits, and reagents needed for Flex Gene Expression.

Experimental Design

Experimental Design & Planning

Flex Gene Expression enables single cell gene expression on precious samples that were previously inaccessible due to logistical challenges in sample handling. Fixation at the point of sample collection preserves fragile biology and greatly streamlines workflows to provide critical insights on more samples.

Prepare single cell/nuclei suspension from fresh samples for fixation or from archived samples by following our Demonstrated Protocols.

Sample Prep

Find a list of probes targeting protein-coding genes in human and mouse transcriptomes for the Flex Gene Expression assay.

Probe Sets

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Sequence your single cell or nuclei library as per our provided recommendations.

Sequencing

Analyze your genome and exome data using our pipelines and visualization tools.

Software Analysis

Chromium X系列单细胞系统

Flex Gene Expression

Overview

User Guides

Workflow Documentation

Additional Applications

Datasets

Videos

What is Flex Gene Expression?

Reveal gene expression and protein expression from the same fixed cell.

Protein

We provide resources from protocols, user guides, and free-to-use analysis tools.

Documentation

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

GEM-X: How to assemble, load and generate GEMs

Chromium Next GEM Tissue Fixation and Dissociation

Chromium Next GEM Single Cell Isolation from FFPE Sections

Find answers to common technical questions, from sample prep through data analysis, about the Flex Gene Expression workflow.

GEM-X Flex Gene Expression	GEM-X Flex v2
GEM-X Flex Sample Preparation v2 Kit, PN-1000781	GEM-X Flex Sample Preparation v2 Kit, PN-1000781
GEM-X Flex Gene Expression Chip Kit, 4 chips PN-1000791	GEM-X Flex Gene Expression Chip Kit, 4 chips PN-1000791
GEM-X Flex GEM & Library Kit, 4 rxns PN-1000782	GEM-X Flex GEM & Library Kit v2, 4 rxns PN-1000918
GEM-X Flex Human/Mouse Transcriptome Probe Kit 16 samples PN-1000785/1000786	GEM-X Flex Human/Mouse Transcriptome Probe Kit v2 16 samples PN-1000900/1000902
GEM-X Flex Human/Mouse Transcriptome Probe Kit 64 samples PN-1000787/1000788	GEM-X Flex Human/Mouse Transcriptome Probe Kit v2 96 samples PN-1000901/1000903
GEM-X Flex Hybridization & Wash Kit, 24 rxns PN-1000789	GEM-X Flex Hybridization & Wash Kit v2, 96 rxns PN-1000904
	GEM-X Flex Barcode Oligo Plate v2 Set A PN-1000894
	GEM-X Flex Barcode Oligo Plate v2 Set B PN-1000897
	GEM-X Flex Barcode Oligo Plate v2 Set C PN-1000898
	GEM-X Flex Barcode Oligo Plate v2 Set D PN-1000899
GEM-X Flex Supplemental Wash Kit, 64 rxns PN-1000828 (optional)	GEM-X Flex Supplemental Wash Kit, 64 rxns PN-1000828 (optional)
	GEM-X Flex Gel Bead & Library Kit v2, 4 rxns PN-1000919 (optional)
	GEM-X Flex Core Reagent Bundle v2, 96 rxns PN-1000937 (optional)
Dual Index Kit TS Set A, 96 rxns PN-1000251	Dual Index Kit TS Set A, 96 rxns PN-1000251
Fixed RNA Feature Barcode Multiplexing Kit 64 rxns PN-1000628*
Dual Index Kit TN Set A 96 rxns PN-1000250*	Dual Index Kit TN Set A 96 rxns PN-1000250*