The Chromium Single Cell Gene Expression Flex workflow produces sequencing-ready libraries for measuring RNA. This Technical Note presents a comparison of sequencing metrics for pooled Chromium Fixed RNA Profiling Gene Expression libraries across Illumina platforms. The expected base percentage profiles and Phred quality scores (Q scores) based on a set of control libraries are described to provide general guidance on the expected range of sequencing metrics on Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.