Describes methods for decalcifying and preparing human and mouse bone tissue samples for use in Xenium v1 In Situ Gene Expression assays. 

Our diverse panel menu of pre-validated biologically relevant targets can be customized with up to 100 additional targets. Standalone custom panels can also be designed for your specific research questions.

Expertly curated, easily customizable in situ panels

Xenium Analyzer

Xenium

Powerful in situ profiling platform using curated panels, powerful visualization software, and easy-to-follow workflow.

Xenium In Situ Gene Expression

Gene Expression

Enables subcellular profiling of 100s of RNA targets from Fresh Frozen or FFPE tissues.

Analysis of Bone Tissue using Xenium v1 In Situ Gene Expression Assay

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for FFPE.

Tissue Prep

The Xenium In Situ enables high-throughput subcellular mapping of 1000s of RNA targets, revealing new insights into cellular structure and function. The platform includes a versatile and easy-to-use instrument, sensitive and specific chemistry, and a diverse menu of customizable panels.

Find resources from protocols to free-to-use analysis tools.

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Xenium Gene Expression.

Experimental Design

Experimental Design & Planning

Learn about the pre-designed Gene Expression panels and engage with 10x design experts to design, order, and manufacture Xenium Custom Genes.

Panel Design

Prepare tissue sections on Xenium slides for on-instrument analysis by following our step-by-step User Guide/Protocols.

Assay

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Analyze your Xenium in situ data using our pipelines and visualization tools.

Software Analysis

Safety Data Sheets

Workflow Documentation

Overview

User Guides

Datasets

Videos

Service Providers

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What is Xenium In Situ Gene Expression?

Access free, publicly available data from a number of different sample types generated with Xenium In Situ solutions.

Xenium In Situ Tissue Preparation

Xenium In Situ Assay Protocols - Best Practices

Find answers to common technical questions, from sample prep through data analysis, about In Situ workflows. 

Analysis of Bone Tissue using Xenium v1 In Situ Gene Expression Assay

Technical Note, CG000816

CG000816_Xenium_Bone_Compatibility_TechNote_RevB.pdf