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Resolving Cell Types as a Function of Read Depth and Cell Number

  • CG000148_10x_Technical_Note_Resolving_Cell_Types_as_Function_of Read_Depth_Cell_Number_RevA.pdf

    Technical Note, CG000148

    CG000148_10x_Technical_Note_Resolving_Cell_Types_as_Function_of Read_Depth_Cell_Number_RevA.pdf

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Technical Note - Resolving Cell Types as a Function of Read Depth and Cell Number

Chromium™ Single Cell Solutions enable gene expression profiling of 100–10,000 cells per reaction. When sequencing costs or capacity are limiting, there is often a trade-off between sequencing a larger cell number (breadth) and sequencing a smaller cell number with more reads (depth). Here, we examine how sequencing depth and cell number influence the detection of major cell types in peripheral blood mononuclear cells (PBMCs).

FOR USE WITH

  • Chromium™ Single Cell 3’ Library & Gel Bead Kit v2, 16 rxns PN-120237
  • Chromium™ Single Cell 3’ Library & Gel Bead Kit v2, 4 rxns PN-120267
  • Chromium™ Single Cell 5’ Library & Gel Bead Kit, 16 rxns PN-1000006
  • Chromium™ Single Cell 5’ Library & Gel Bead Kit, 4 rxns PN-1000014
  • Chromium™ Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005
  • Chromium™ Single Cell A Chip Kit, 48 rxns PN-120236
  • Chromium™ Single Cell A Chip Kit, 16 rxns PN-1000009
  • Chromium™ i7 Multiplex Kit, 96 rxns PN-120262
Document Type
Technical Note

Last Modified
September 25, 2019