10x Genomics Support/Single Cell Gene Expression Flex/Documentation/Sequencing/

Sequencing Metrics & Base Composition of Chromium Fixed RNA Profiling Libraries

  • CG000677_Flex_Gene_Expression_SeqMetrics_BaseComposition_Rev_B.pdf

    Technical Note, CG000677

    CG000677_Flex_Gene_Expression_SeqMetrics_BaseComposition_Rev_B.pdf

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The Chromium Single Cell Gene Expression Flex workflow produces sequencing-ready libraries for measuring RNA. This Technical Note presents a comparison of sequencing metrics for pooled Chromium Fixed RNA Profiling Gene Expression libraries across Illumina platforms. The expected base percentage profiles and Phred quality scores (Q scores) based on a set of control libraries are described to provide general guidance on the expected range of sequencing metrics on Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.

For Use With:

  • Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 1 BC PN-1000474
  • Chromium Fixed RNA Kit, Mouse Transcriptome, 4 rxns x 1 BC PN-1000495
  • Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 4 BC PN-1000475 | 4 rxns x 16 BC PN-1000476
  • Chromium Fixed RNA Kit, Mouse Transcriptome, 4 rxns x 4 BC PN-1000496 | 4 rxns x 16 BC PN-1000497
  • Chromium Next GEM Chip Q Single Cell Kit, 48 rxns PN-1000418 / 16 rxns PN-1000422
  • Dual Index Kit TS Set A 96 rxns PN-1000251
Document Type
Technical Note

Last Modified
September 20, 2023