https://downloads.ctfassets.net/an68im79xiti/6LZ0VZWgl6zRNDdoszI35P/77099a10fa829e97013abe1f6c2e730b/CG000549_VisiumCytAssistTrainingUserGuide_RevA.pdf

Provides instructions for training Visium CytAssist users.

Visium CytAssist Spatial Training Kit User Guide

Visium CytAssist

Visium CytAssist Spatial Gene and Protein Expression simultaneously measures whole transcriptome and highly multiplexed protein expression of FFPE tissue sections in a spatially resolved manner by mapping gene and protein expression over a high-resolution microscope image of the H&E- or IF-stained tissue.

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Spatial Gene and Protein Expression protocols

Experimental Design

Experimental Planning & Design

Tissue Prep

Deparaffinize, decrosslink, and stain tissue

Tissue Staining

For imaging hardware, general image acquisition and analysis guidelines, and example images

Imaging

Find a list of probes targeting protein-coding genes in human transcriptome for the Spatial Gene and Protein Expression assay

Probe Set

Find a list of proteins detected by the human protein panel for the Spatial Gene and Protein Expression assay

Protein Panel

Construct sequencing-ready Visium spatial libraries from your FFPE tissue sections

Library Construction

Refer to instrument-specific documents for seamless instrument operation

Instruments

Sequence your Spatial Gene and Protein Expression libraries as per our provided recommendations

Sequencing

Analyze your Visium spatial data using our pipelines and visualization tools.

Software Analysis

Spatial Gene and Protein Expression

Overview

Documentation

Datasets

Videos

CytAssist Spatial Gene and Protein Expression

What is Visium CytAssist Spatial Gene and Protein Expression?

Find resources, from protocols to free-to-use analysis tools. Visium CytAssist Spatial Gene and Protein Expression currently supports immune cell-containing human FFPE samples.

Official datasets are coming soon. Access free, publicly available data from a number of different sample types generated with Visium Spatial solutions.

Videos - CytAssist Spatial Gene and Protein Expression

Visium CytAssist Instrument

Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.

Visium CytAssist Spatial Training Kit User Guide

User Guide, CG000549

CG000549_VisiumCytAssistTrainingUserGuide_RevC.pdf