GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species

Universal 3'

Chromium X Series

Chromium

Advanced system for multiomic analysis of hundreds to millions of cells.

Gene Expression

Epigenomic Profiling

Immune Profiling

Protein co-detection

3' gene expression profiling at scale with single cell resolution.

GEM-X technology-powered assays with whole transcriptome coverage to analyze immune diversity 

Universal 5'

5' gene expression alongside V(D)J repertoire profiling and antigen specificity of T and B cells.

Single cell profiling of open chromatin and 3' gene expression from the same nucleus

Epi Multiome

Combined profiling of 3' gene expression and chromatin accessibility from the same nucleus.

Plate-based sample handling brings unprecedented scale to highly sensitive single cell profiling

Flex

Fixed RNA Profiling assay for comprehensive probe-based gene expression profiling with single cell resolution.

Visium CytAssist

Visium

Compact instrument designed to simplify the Visium workflow by facilitating the transfer of analytes from standard glass slides to Visium slides.

CytAssist Spatial Gene Expression

Probe-based assay allowing simultaneous analysis of molecular and imaging data from FFPE, Fresh Frozen, and Fixed Frozen tissue sections.

HD Spatial Gene Expression

Whole transcriptome probe-based gene expression assay at single cell scale, compatible with FFPE, Fresh Frozen, and Fixed Frozen tissue sections

Combine whole transcriptome spatial analysis with high-plex protein detection using NGS in the same tissue section. Gain a deeper, more holistic understanding of tissue organization, using Visium CytAssist to expand sample access and ease workflows.

Immunofluorescence protein co-detection

Visium CytAssist Gene and Protein Expression

Tissue profiling with transcriptomics and <em>protein co-detection</em>

CytAssist Spatial Gene and Protein Expression

Simultaneous analysis of RNA and protein expression of FFPE tissues, using probe-based chemistry and tagged antibodies.

Spatial Gene Expression for Fresh Frozen

Poly-A based assay allowing simultaneous analysis of molecular and imaging data from fresh frozen tissue sections.

RNase Inhibitor Safety Data Sheets

Construct a sequencing-ready Visium spatial library from your fresh frozen tissue sections following our step-by-step User Guide.

Library Construction

Visium Spatial Gene Expression measures the whole transcriptome of intact fresh frozen sections in a spatially resolved manner by mapping gene expression over a high-resolution microscope image of the H&E- or IF-stained tissue.

Find resources from protocols to free-to-use analysis tools.

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial protocol.

Experimental Design

Experimental Design & Planning

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Tissue Preparation Guide.

Tissue Prep

Methanol fix, stain, and image tissue following our Demonstrated Protocol.

Tissue Staining

Optimize permeabilization conditions for your fresh frozen tissue sections by following our step-by step User Guide.

Tissue Optimization

For imaging hardware, general image acquisition and analysis guidelines, and example images, refer to our Technical Note.

Imaging

Use pre-designed panels for targeted gene expression, followed by sequencing and data analysis.

Targeted

Targeted Gene Expression (Optional)

Sequence your Visium spatial library as per our provided recommendations.

Sequencing

Analyze your Visium spatial data using our pipelines and visualization tools.

Software Analysis

Workflow Documentation

Visium Spatial Gene Expression is a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context in FFPE or fresh frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research.

Map the whole transcriptome within the <em>tissue context</em>

Spatial Gene Expression

Spatial gene expression profiling and imaging data of fresh frozen or FFPE tissues.

Overview

User Guides

Datasets

Videos

Publications

What is Spatial Gene Expression for Fresh Frozen?

Access free, publicly available data from a number of different sample types generated with Visium spatial solutions

Explore how-to videos and training modules.

Visium Tissue Preparation Guide

Visium Tissue Optimization

Visium Gene Expression

Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.

RNase Inhibitor Safety Data Sheets

SDS

1000887_RNase_Inhibitor_40x_SDS_Ed1.pdf