https://assets.ctfassets.net/an68im79xiti/4cFfVJH95mgYeSIcEAUE6Y/2699fe2d0bc8c631f00e9020a1927aa8/CG00054_SamplePrepDemonstratedProtocol_-_CultCells_RevB.pdf

Outlines best practices for preparing single cell suspensions from cultured cell lines.

Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing

Prepare optimal single cell/nuclei suspension by following our Demonstrated Protocols.

Sample Prep

Chromium Universal 5' Gene Expression allows analysis of full-length, paired B-cell or T-cell receptor (BCR/TCR) sequences, surface protein expression, antigen specificity, and 5’ gene expression, all from a single cell. Scale up with a high-throughput solution, or focus on key genes with targeted gene expression panels.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Single Cell Immune Profiling.

Experimental Design

Experimental Design & Planning

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Use pre-designed or custom panels for targeted gene expression, followed by sequencing and data analysis.

Targeted

Targeted Gene Expression (Optional)

Sequence your single cell library as per our provided recommendations.

Sequencing

Analyze your single cell data using our pipelines, visualization, and Cloud Analysis tools.

Software Analysis

Chromium X系列单细胞系统

Universal 5' Gene Expression

Overview

User Guides

Workflow Documentation

Additional Applications

Datasets

Videos

Publications

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Chromium Universal 5' Gene Expression allows analysis of full-length, paired B-cell or T-cell receptor (BCR/TCR) sequences, surface protein expression, and 5’ gene expression, all from a single cell.

What is Universal 5' Gene Expression?

Measure single cell gene expression, cell surface protein expression, and optionally antigen specificity simultaneously.

Cell Surface Protein

Assess the effects of CRISPR perturbations alongside single cell gene expression.

CRISPR Screening

Enables molecular profiling of 2,000–20,000 cells/sample with simultaneous detection of full length, paired immune receptor sequences, gene and protein expression, and antigen specificity.

High Throughput (HT)

Map antigens to discover novel antigen-specific Ig or T-cell receptors.

Barcode Enabled Antigen Mapping (BEAM)

We provide resources from protocols, user guides, and free-to-use analysis tools.

Documentation

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

GEM-X: How to assemble, load and generate GEMs

GEM-X OCM: How to assemble, load and generate multiplex GEMs

Chromium Next GEM Single Cell 5' v2 Dual Index

Find answers to common technical questions, from sample prep through data analysis, about Chromium single cell workflows.

Q&As

Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.

Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing

Demonstrated Protocol, CG00054

CG00054_SamplePrepDemonstratedProtocol - CultCells_RevB.pdf